Investigating genomic, proteomic, and post-transcriptional regulation profiles in colorectal cancer: a comparative study between primary tumors and associated metastases.

ABSTRACT:

Introduction

Approximately 50% of patients with primary colorectal carcinoma develop liver metastases. This study investigates the possible molecular discrepancies between primary colorectal cancer (pCRC) and their respective metastases.

Methods

A total of 22 pairs of pCRC and metastases were tested. Mutation profiling of 26 cancer-associated genes was undertaken in 22/22primary-metastasis tumour pairs using next-generation sequencing, whilst the expression of a panel of six microRNAs (miRNAs) was investigated using qPCRin 21/22 pairs and 22 protein biomarkers was tested using Reverse Phase Protein Array (RPPA)in 20/22 patients' tumour pairs.

Results

Among the primary and metastatic tumours the mutation rates for the individual genes are as follows:TP53 (86%), APC (44

SUBMITTER: Ham-Karim H 

PROVIDER: S-EPMC10478430 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature