Functional evolutionary convergence of long noncoding RNAs involved in embryonic development.

ABSTRACT: Long noncoding RNAs have been identified in most vertebrates, but the functional characterization of these molecules is challenging, mainly due to the lack of linear sequence homology between species. In this work, we aimed to find functional evolutionary convergent lncRNAs involved in development by screening of k-mer content (nonlinear similarity) and secondary structure-based approaches combining in silico, in vitro and in vivo validation analysis. From the Madagascar gecko genes, we have found a non-orthologous lncRNA with a similar k-mer content and structurally concordant with the human lncRNA EVX1AS. Analysis of function-related characteristics together with locus-specific targeting of human EVX1AS and gecko EVX1AS-like (i.e., CRISPR Display) in human neuroepithelial cells and chick

SUBMITTER: Olazagoitia-Garmendia A 

PROVIDER: S-EPMC10480150 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature