Tensin-3 is involved in osteogenic versus adipogenic fate of human bone marrow stromal cells.

ABSTRACT:

Background

The tightly controlled balance between osteogenic and adipogenic differentiation of human bone marrow-derived stromal cells (BMSCs) is critical to maintain bone homeostasis. Age-related osteoporosis is characterized by low bone mass with excessive infiltration of adipose tissue in the bone marrow compartment. The shift of BMSC differentiation from osteoblasts to adipocytes could result in bone loss and adiposity.

Methods

TNS3 gene expression during osteogenic and adipogenic differentiation of BMSCs was evaluated by qPCR and Western blot analyses. Lentiviral-mediated knockdown or overexpression of TNS3 was used to assess its function. The organization of cytoskeleton was examined by immunofluorescent staining at multiple time points. The role of TNS3 and its domain

SUBMITTER: Zhang S 

PROVIDER: S-EPMC10480249 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature