Clinical Relevance of Patient-Derived Organoid of Surgically Resected Lung Cancer as an In Vitro Model for Biomarker and Drug Testing.

ABSTRACT:

Introduction

Lung tumor organoids (LTOs) have attracted attention as in vitro preclinical models; however, their clinical and experimental applications have not been fully established.

Methods

We attempted to establish LTOs from resected specimens of patients with lung cancer who underwent lung resection. Clinicopathologic characteristics related to the establishment of LTOs were evaluated. Histologic assessment and genetic analysis were conducted for both LTOs and their parental tumors. Organoid-derived xenografts were generated in immunocompetent mice. Drug sensitivity was assessed using cell proliferation assays.

Results

We established 53 LTOs from 79 lung cancer samples, including 10 long-term culture models. The establishment rate was significantly lower i

SUBMITTER: Koga T 

PROVIDER: S-EPMC10480534 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature