Development of a new recombinant antibody, selected by phage-display technology from a celiac patient library, for detection of gluten in foods.

ABSTRACT: Gluten, a group of ethanol-soluble proteins present in the endosperm of cereals, is extensively used in the food industry due to its ability to improve dough properties. However, gluten is also associated with a range of gluten-related diseases (GRDs), such as wheat allergies, celiac disease, and gluten intolerance. The recommended treatment for GRDs patients is a gluten-free diet. To monitor adherence to this diet, it is necessary to develop gluten-detection systems in food products. Among the available methods, immunodetection systems are the most popular due to their simplicity, reproducibility, and accuracy. The aim of this study was to generate novel high-affinity antibodies against gluten to be used as the primary reactant in an enzyme-linked immunosorbent assay (ELISA) test. These a

SUBMITTER: Garcia-Calvo E 

PROVIDER: S-EPMC10480589 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature