Microglial INPP5D limits plaque formation and glial reactivity in the PSAPP mouse model of Alzheimer's disease.

ABSTRACT:

Introduction

The inositol polyphosphate-5-phosphatase D (INPP5D) gene encodes a dual-specificity phosphatase that can dephosphorylate both phospholipids and phosphoproteins. Single nucleotide polymorphisms in INPP5D impact risk for developing late onset sporadic Alzheimer's disease (LOAD).

Methods

To assess the consequences of inducible Inpp5d knockdown in microglia of APP^KM670/671NL /PSEN1^Δexon9 (PSAPP) mice, we injected 3-month-old Inpp5d^fl/fl /Cx3cr1^CreER/+ and PSAPP/Inpp5d^fl/fl /Cx3cr1^CreER/+ mice with either tamoxifen (TAM) or corn oil (CO) to induce recombination.

Results

At age 6 months, we found that the percent area of 6E10⁺ deposits and plaque-associated microglia in Inpp5d knockdow

SUBMITTER: Castranio EL 

PROVIDER: S-EPMC10481344 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature