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Neurogenesis redirects β-catenin from adherens junctions to the nucleus to promote axonal growth.


ABSTRACT: Here, we show that, in the developing spinal cord, after the early Wnt-mediated Tcf transcription activation that confers dorsal identity to neural stem cells, neurogenesis redirects β-catenin from the adherens junctions to the nucleus to stimulate Tcf-dependent transcription in a Wnt-independent manner. This new β-catenin activity regulates genes implicated in several aspects of contralateral axon growth, including axon guidance and adhesion. Using live imaging of ex-vivo chick neural tube, we showed that the nuclear accumulation of β-catenin and the rise in Tcf-dependent transcription both initiate before the dismantling of the adherens junctions and remain during the axon elongation process. Notably, we demonstrated that β-catenin activity in post-mitotic cells depends on TCF7L2 and is central to spinal commissural axon growth. Together, our results reveal Wnt-independent Tcf/β-catenin regulation of genes that control the growth and guidance of commissural axons in chick spinal cord.

SUBMITTER: Herrera A 

PROVIDER: S-EPMC10482005 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature

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Neurogenesis redirects β-catenin from adherens junctions to the nucleus to promote axonal growth.

Herrera Antonio A   Menendez Anghara A   Ochoa Andrea A   Bardia Lídia L   Colombelli Julien J   Pons Sebastian S  

Development (Cambridge, England) 20230824 16


Here, we show that, in the developing spinal cord, after the early Wnt-mediated Tcf transcription activation that confers dorsal identity to neural stem cells, neurogenesis redirects β-catenin from the adherens junctions to the nucleus to stimulate Tcf-dependent transcription in a Wnt-independent manner. This new β-catenin activity regulates genes implicated in several aspects of contralateral axon growth, including axon guidance and adhesion. Using live imaging of ex-vivo chick neural tube, we  ...[more]

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