Induction of liver-resident memory T cells and protection at liver-stage malaria by mRNA-containing lipid nanoparticles.

ABSTRACT: Recent studies have suggested that CD8⁺ liver-resident memory T (T_RM) cells are crucial in the protection against liver-stage malaria. We used liver-directed mRNA-containing lipid nanoparticles (mRNA-LNPs) to induce liver T_RM cells in a murine model. Single-dose intravenous injections of ovalbumin mRNA-LNPs effectively induced antigen-specific cytotoxic T lymphocytes in a dose-dependent manner in the liver on day 7. T_RM cells (CD8⁺ CD44^hi CD62L^lo CD69⁺ KLRG1^-) were induced 5 weeks after immunization. To examine the protective efficacy, mice were intramuscularly immunized with two doses of circumsporozoite protein mRNA-LNPs at 3-week intervals and challenged with sporozoites of Plasmodium b

SUBMITTER: Nakamae S 

PROVIDER: S-EPMC10482405 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature