Comparative Transcriptomic Analyses of a Vero Cell Line in Suspension versus Adherent Culture Conditions.

ABSTRACT: The Vero cell line is the most used continuous cell line for viral vaccine manufacturing. Its anchorage-dependent use renders scaling up challenging and operations very labor-intensive which affects cost effectiveness. Thus, efforts to adapt Vero cells to suspension cultures have been invested, but hurdles such as the long doubling time and low cell viability remain to be addressed. In this study, building on the recently published Vero cell line annotated genome, a functional genomics analysis of the Vero cells adapted to suspension is performed to better understand the genetic and phenotypic switches at play during the adaptation of Vero cells from anchorage-dependent to suspension cultures. Results show downregulation of the epithelial-to-mesenchymal transition (EMT) pathway, highlighti

SUBMITTER: Sene MA 

PROVIDER: S-EPMC10482560 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature