Gasdermin D-mediated keratinocyte pyroptosis as a key step in psoriasis pathogenesis.

ABSTRACT: Gasdermin D (GSDMD)-mediated pyroptosis has a significant pro-inflammation characteristic due to dramatic secretion of pro-inflammatory substances. However, its role remains unclear in psoriasis as one chronic inflammatory skin disorder with high prevalence. We found that N-terminal GSDMD (N-GSDMD) was aberrantly expressed in epidermis of skin lesion in psoriasis patients and imiquimod-induced psoriasis-like dermatitis (IIPLD) mice. In epidermis of IIPLD mice and M5 (simulating psoriatic inflammatory challenge)-treated keratinocytes cultured in vitro, cleavage products of caspase-1, GSDMD and IL-1β were increased. M5-stimulated keratinocyte presented typical pyroptosis morphology accompanied with PI-staining. Gsdmd^-/- keratinocytes could not present pyroptosis morphology while s

SUBMITTER: Lian N 

PROVIDER: S-EPMC10482869 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature