A novel auxin-inducible degron system for rapid, cell cycle-specific targeted proteolysis.

ABSTRACT: The discrimination of protein biological functions in different phases of the cell cycle is limited by the lack of experimental approaches that do not require pre-treatment with compounds affecting the cell cycle progression. Therefore, potential cycle-specific biological functions of a protein of interest could be biased by the effects of cell treatments. The OsTIR1/auxin-inducible degron (AID) system allows "on demand" selective and reversible protein degradation upon exposure to the phytohormone auxin. In the current format, this technology does not allow to study the effect of acute protein depletion selectively in one phase of the cell cycle, as auxin similarly affects all the treated cells irrespectively of their proliferation status. Therefore, the AID system requires coupling with

SUBMITTER: Capece M 

PROVIDER: S-EPMC10482871 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature