Monitoring live mitochondrial metabolism in real-time using NMR spectroscopy.

ABSTRACT: Investigation of mitochondrial metabolism is gaining increased interest owing to the growing recognition of the role of mitochondria in health and numerous diseases. Studies of isolated mitochondria promise novel insights into the metabolism devoid of confounding effects from other cellular organelles such as cytoplasm. This study describes the isolation of mitochondria from mouse skeletal myoblast cells (C2C12) and the investigation of live mitochondrial metabolism in real-time using isotope tracer-based NMR spectroscopy. [3-¹³ C₁ ]pyruvate was used as the substrate to monitor the dynamic changes of the downstream metabolites in mitochondria. The results demonstrate an intriguing phenomenon, in which lactate is produced from pyruvate inside the mitochondria and the r

SUBMITTER: Nagana Gowda GA 

PROVIDER: S-EPMC10483017 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature