Extended lifespan and improved genome stability in HepaRG-derived cell lines through reprogramming by high-density stress.

ABSTRACT: The characteristics and fate of cancer cells partly depend on their environmental stiffness, i.e., the local mechanical cues they face. HepaRG progenitors are liver carcinoma cells exhibiting transdifferentiation properties; however, the underlying mechanisms remain unknown. To evaluate the impact of external physical forces mimicking the tumor microenvironment, we seeded them at very high density for 20 h, keeping the cells round and unanchored to the substrate. Applied without corticoids, spatial confinement due to very high density induced reprogramming of HepaRG cells into stable replicative stem-like cells after replating at normal density. Redifferentiation of these stem-like cells into cells very similar to the original HepaRG cells was then achieved using the same stress but in the

SUBMITTER: Brun C 

PROVIDER: S-EPMC10483629 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature