Quartet protein reference materials and datasets for multi-platform assessment of label-free proteomics.

ABSTRACT:

Background

Quantitative proteomics is an indispensable tool in life science research. However, there is a lack of reference materials for evaluating the reproducibility of label-free liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based measurements among different instruments and laboratories.

Results

Here, we develop the Quartet standard as a proteome reference material with built-in truths, and distribute the same aliquots to 15 laboratories with nine conventional LC-MS/MS platforms across six cities in China. Relative abundance of over 12,000 proteins on 816 mass spectrometry files are obtained and compared for reproducibility among the instruments and laboratories to ultimately generate proteomics benchmark datasets. There is a wide dynamic range of proteomes

SUBMITTER: Tian S 

PROVIDER: S-EPMC10483797 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature