Non-spatial and spatial heterogeneity revealed a suppressive immune feature of Siglec-15 in lung adenocarcinomas.

ABSTRACT:

Background

Sialic acid-binding immunoglobulin-like lectin-15 (Siglec-15) has emerged as a novel immunotherapy candidate, which deserves a comprehensive investigation in lung adenocarcinoma (LUAD).

Methods

Multiplex fluorescence-based immunohistochemistry was conducted to assess Siglec-15 expression and tumor-infiltrating immune cells in LUAD from Tianjin cohort, with validation cohorts Xinchao 04 and 07.

Results

This study revealed that Siglec-15 was positively correlated with CD8⁺ T cells and tumor-associated macrophages (TAMs) infiltration, but CD8⁺ T cells were mostly infiltrated in the stroma area, not in the tumor area. Spatially, fewer CD8⁺ T cells surrounded Siglec-15⁺ tumor cells in PD-L1^- cells, and more

SUBMITTER: Li B 

PROVIDER: S-EPMC10483852 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature