Large-scale mapping and mutagenesis of human transcriptional effector domains.

ABSTRACT: Human gene expression is regulated by more than 2,000 transcription factors and chromatin regulators^1,2. Effector domains within these proteins can activate or repress transcription. However, for many of these regulators we do not know what type of effector domains they contain, their location in the protein, their activation and repression strengths, and the sequences that are necessary for their functions. Here, we systematically measure the effector activity of more than 100,000 protein fragments tiling across most chromatin regulators and transcription factors in human cells (2,047 proteins). By testing the effect they have when recruited at reporter genes, we annotate 374 activation domains and 715 repression domains, roughly 80% of which are new and have not been previousl

SUBMITTER: DelRosso N 

PROVIDER: S-EPMC10484233 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature