Acquiring bifidobacteria species from formula-fed and breast-fed newborns: identifying, quantifying and creating an antibiogram.

ABSTRACT: After examining the Bifidobacterium spp. population in faeces samples from breast-fed and formula-fed infants, an antibiogram was created. The prevalence of Bifidobacterium spp. in faeces was determined using common bacterial growth media, including Man Rogos Sharpe (MRS), Brain Heart Infusion (BHI), Luria Bertani (LB) broth and Bifidobacteria agar. According to the findings, formula-fed babies had a low population of Bifidobacterium spp. in their stools while breast-fed babies had a high population. By using phylogenetic analysis of the 16S rRNA and xfp (xylose/fructose 6-phosphate phosphoketolase) genes, and RFLP mapping of Bifidobacterium isolates, it was possible to identify a new and unique Bifidobacterium species. The intensity of the reddish

SUBMITTER: Kammara R 

PROVIDER: S-EPMC10484311 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature