African ZIKV lineage fails to sustain infectivity in an in vitro mimetic urban cycle.

ABSTRACT: Zika virus (ZIKV) is an arbovirus maintained in nature in two distinct cycles of transmission: urban and sylvatic. Each cycle includes specific vertebrate and invertebrate hosts, and through alternate infections, a conserved consensus sequence is maintained that might vary depending on the cycle. The current study aimed to investigate the ability of ZIKV^AF and ZIKV^BR to maintain an infectious cycle by alternating passages in cells mimicking the urban (UC) and semi-sylvatic (SC) cycles. The complete genome of the original inoculum and the last passages for each cycle were sequenced by Sanger. Ten passages were performed, as planned, for ZIKV^BR UC, ZIKV^AF SC, and ZIKV^BR SC. ZIKV^BR SC showed significant variation in viral titer

SUBMITTER: Molina BF 

PROVIDER: S-EPMC10484821 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature