Production, purification, and characterization of cold-active lipase from the psychrotroph Pseudomonas sp. A6.

ABSTRACT: Cold-active lipases are presently employed extensively in the detergent, chemical intermediate, fine chemical, food, and pharmaceutical industries. Seven cold-adaptive bacteria were isolated from the Mediterranean Sea near Alexandria, Egypt, and tested for their ability to produce cold-active lipase, with the highest activity at 10 °C. The most potent isolate was Pseudomonas sp. A6. To determine the most important variables, the bacterium was exposed to a necessary medium component and environmental factor screening using a single factor-at-a-time approach, followed by a multifactorial Plackett-Burman design strategy. After purification and characterization, the optimal activity levels for the cold-active lipase were figured out. Inoculation of Pseudomonas A6 under near optimum conditions

SUBMITTER: Abdella B 

PROVIDER: S-EPMC10484855 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature