Ontology highlight
ABSTRACT:
SUBMITTER: Ye Y
PROVIDER: S-EPMC10485044 | biostudies-literature | 2023 Sep
REPOSITORIES: biostudies-literature
Ye Yansheng Y Tyndall Erin R ER Bui Van V Bewley Maria C MC Wang Guifang G Hong Xupeng X Shen Yang Y Flanagan John M JM Wang Hong-Gang HG Tian Fang F
Nature communications 20230907 1
Autophagosome formation, a crucial step in macroautophagy (autophagy), requires the covalent conjugation of LC3 proteins to the amino headgroup of phosphatidylethanolamine (PE) lipids. Atg3, an E2-like enzyme, catalyzes the transfer of LC3 from LC3-Atg3 to PEs in targeted membranes. Here we show that the catalytically important C-terminal regions of human Atg3 (hAtg3) are conformationally dynamic and directly interact with the membrane, in collaboration with its N-terminal membrane curvature-sen ...[more]