The complete ITS2 barcoding region for Strongylus vulgaris and Strongylus edentatus.

ABSTRACT: Gastrointestinal nematode parasites are of major concern for horses, where Strongylus vulgaris is considered the most pathogenic among the Strongylus species. Diagnosis of S. vulgaris infections can be determined with next generation sequencing techniques, which are inherently dependent on reference sequences. The best marker for parasitic nematodes is internal transcribed spacer 2 (ITS2) and we provide the first complete ITS2 sequences from five morphologically identified S. vulgaris and additional sequences from two S. edentatus. These sequences have high similarity to already published partial sequences and amplicon sequence variants (ASV) based on next generation sequencing (NGS). The ITS2 sequences from S. vulgaris matched available partial ITS2 sequences and the full ASVs, whereas th

SUBMITTER: Halvarsson P 

PROVIDER: S-EPMC10485102 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature