Mycobacterium tuberculosis impairs human memory CD4⁺ T cell recognition of M2 but not M1-like macrophages.

ABSTRACT: Direct recognition of Mycobacterium tuberculosis (Mtb)-infected cells is required for protection by CD4⁺ T cells. While impaired T cell recognition of Mtb-infected macrophages was demonstrated in mice, data are lacking for humans. Using T cells and monocyte-derived macrophages (MDMs) from individuals with latent Mtb infection (LTBI), we quantified the frequency of memory CD4⁺ T cell activation in response to autologous MDMs infected with virulent Mtb. We observed robust T cell activation in response to Mtb infection of M1-like macrophages differentiated using GM-CSF, while M2-like macrophages differentiated using M-CSF were poorly recognized. However, non-infected GM-CSF and M-CSF MDMs loaded with exogenous antigens elicited similar CD4⁺ T cell activ

SUBMITTER: Gail DP 

PROVIDER: S-EPMC10485162 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature