Hydrogel-based 3D human iPSC-derived neuronal culture for the study of rabies virus infection.

ABSTRACT:

Background

Rabies is a highly fatal infectious disease that poses a significant threat to human health in developing countries. In vitro study-based understanding of pathogenesis and tropism of different strains of rabies virus (RABV) in the central nervous system (CNS) is limited due to the lack of suitable culture models that recapitulate the complex communication pathways among host cells, extracellular matrices, and viruses. Therefore, a three-dimensional (3D) cell culture that mimics cell-matrix interactions, resembling in vivo microenvironment, is necessary to discover relevant underlying mechanisms of RABV infection and host responses.

Methods

The 3D collagen-Matrigel hydrogel encapsulating hiPSC-derived neurons for RABV infection was developed and characterized based

SUBMITTER: Muangsanit P 

PROVIDER: S-EPMC10485840 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature