Engineering a replication-incompetent viral vector for the delivery of therapeutic RNA in crustaceans.

ABSTRACT: Viral disease pandemics are a major cause of economic losses in crustacean farming worldwide. While RNA interference (RNAi)-based therapeutics have shown promise at a laboratory scale, without an effective oral delivery platform, RNA-based therapy will not reach its potential against controlling viral diseases in crustaceans. Using a reverse-engineered shrimp RNA virus, Macrobrachium rosenbergii nodavirus (MrNV), we have developed a shrimp viral vector for delivering an engineered RNA cargo. By replacing the RNA-dependent RNA polymerase (RdRp) protein-coding region of MrNV with a cargo RNA encoding green fluorescent protein (GFP) as a proof-of-concept, we generated a replication-incompetent mutant MrNV^(ΔRdRp) carrying the GFP RNA cargo resulting in MrNV^(ΔRdRp)-

SUBMITTER: Alenton RRR 

PROVIDER: S-EPMC10485883 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature