Positron emission tomography imaging of the sodium iodide symporter senses real-time energy stress in vivo.

ABSTRACT:

Background

Tissue environment is critical in determining tumour metabolic vulnerability. However, in vivo drug testing is slow and waiting for tumour growth delay may not be the most appropriate endpoint for metabolic treatments. An in vivo method for measuring energy stress would rapidly determine tumour targeting in a physiologically relevant environment. The sodium-iodide symporter (NIS) is an imaging reporter gene whose protein product co-transports sodium and iodide, and positron emission tomography (PET) radiolabelled anions into the cell. Here, we show that PET imaging of NIS-mediated radiotracer uptake can rapidly visualise tumour energy stress within minutes following in vivo treatment.

Methods

We modified HEK293T human embryonic kidney cells, and A549 and H358 lung

SUBMITTER: Dzien P 

PROVIDER: S-EPMC10486058 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature