In vitro-generated human muscle reserve cells are heterogeneous for Pax7 with distinct molecular states and metabolic profiles.

ABSTRACT:

Background

The capacity of skeletal muscles to regenerate relies on Pax7⁺ muscle stem cells (MuSC). While in vitro-amplified MuSC are activated and lose part of their regenerative capacity, in vitro-generated human muscle reserve cells (MuRC) are very similar to quiescent MuSC with properties required for their use in cell-based therapies.

Methods

In the present study, we investigated the heterogeneity of human MuRC and characterized their molecular signature and metabolic profile.

Results

We observed that Notch signaling is active and essential for the generation of quiescent human Pax7⁺ MuRC in vitro. We also revealed, by immunofluorescence and flow cytometry, two distinct subpopulations of MuRC distinguished by their relative Pax7 expression.

SUBMITTER: Bouche A 

PROVIDER: S-EPMC10486062 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature