A novel method for in vitro culture and expansion of nonhuman primate B cells.

ABSTRACT:

Background

Given the role of B cells in sensitization and antibody-mediated rejection pathogenesis, the ability to identify, isolate, and study B cells in vitro is critical for understanding these processes and developing novel therapeutics. While in vivo nonhuman primate models have been used to this end, an in vitro nonhuman primate model of B cell activation and proliferation has not been developed.

Methods

CD20⁺ B cells and CD3⁺ T cells were isolated using magnetic bead separation from the peripheral blood of naive and skin allograft sensitized nonhuman primates. Allogeneic B and T cells were co-cultured in plates pre-coated with murine stromal cells engineered to express human CD40L and stimulated with cytokines. Cells and supernatants were harvest

SUBMITTER: DeLaura I 

PROVIDER: S-EPMC10486248 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature