Whole mount of adult ear skin as a model to study vascular malformations.

ABSTRACT:

Background

Genetic analysis in human patients has linked mutations in PIK3CA, the catalytic subunit of PI-3'Kinase, to sporadic incidences of vascular malformations.

Methods

We have developed a mouse model with inducible and endothelial-specific expression of PIK3CA^H1047R , resulting in the development of vascular malformations. Systemic induction of this mutation in adult mice results in rapid lethality, limiting our ability to track and study these lesions; therefore, we developed a topical and local induction protocol using the active metabolite of tamoxifen, 4OH-T, on the ear skin of adults.

Results

This approach allows us to successfully model the human disease in a mature and established vascular bed and track the development of vascular malformations

SUBMITTER: Decker-Rockefeller B 

PROVIDER: S-EPMC10486327 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature