Lysophosphatidic Acid Signalling Regulates Human Sperm Viability via the Phosphoinositide 3-Kinase/AKT Pathway.

ABSTRACT: Lysophosphatidic acid (LPA) signalling is essential for maintaining germ cell viability during mouse spermatogenesis; however, its role in human spermatozoa is unknown. We previously demonstrated that peroxiredoxin 6 (PRDX6) calcium-independent phospholipase A₂ (iPLA₂) releases lysophospholipids such as LPA or arachidonic acid (AA) and that inhibiting PRDX6 iPLA₂ activity impairs sperm cell viability. The exogenous addition of LPA bypassed the inhibition of PRDX6 iPLA₂ activity and maintained the active phosphoinositide 3-kinase (PI3K)/AKT pathway. Here, we aimed to study PI3K/AKT pathway regulation via LPA signalling and protein kinases in maintaining sperm viability. The localization of LPARs in human spermatozoa was determined using immunocyto

SUBMITTER: Liao HY 

PROVIDER: S-EPMC10486690 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature