Morphology of Neutrophils during Their Activation and NETosis: Atomic Force Microscopy Study.

ABSTRACT: Confocal microscopy and fluorescence staining of cellular structures are commonly used to study neutrophil activation and NETosis. However, they do not reveal the specific characteristics of the neutrophil membrane surface, its nanostructure, and morphology. The aim of this study was to reveal the topography and nanosurface characteristics of neutrophils during activation and NETosis using atomic force microscopy (AFM). We showed the main stages of neutrophil activation and NETosis, which include control cell spreading, cell fragment formation, fusion of nuclear segments, membrane disruption, release of neutrophil extracellular traps (NETs), and final cell disintegration. Changes in neutrophil membrane nanosurface parameters during activation and NETosis were quantified. It was shown that

SUBMITTER: Sergunova V 

PROVIDER: S-EPMC10486724 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature