Improved Method for Dental Pulp Stem Cell Preservation and Its Underlying Cell Biological Mechanism.

ABSTRACT: Dental pulp stem cells (DPSCs) are considered a valuable cell source for regenerative medicine because of their high proliferative potential, multipotency, and availability. We established a new cryopreservation method (NCM) for collecting DPSCs, in which the tissue itself is cryopreserved and DPSCs are collected after thawing. We improved the NCM and developed a new method for collecting and preserving DPSCs more efficiently. Dental pulp tissue was collected from an extracted tooth, divided into two pieces, sandwiched from above and below using cell culture inserts, and cultured. As a result, the cells in the pulp tissue migrated vertically over time and localized near the upper and lower membranes over 2-3 days. With regard to the underlying molecular mechanism, SDF1 was predominantly in

SUBMITTER: Takeshita-Umehara M 

PROVIDER: S-EPMC10486868 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature