Simultaneous editing of two DMR6 genes in grapevine results in reduced susceptibility to downy mildew.

ABSTRACT: The reduction of pesticide treatments is of paramount importance for the sustainability of viticulture, and it can be achieved through a combination of strategies, including the cultivation of vines (Vitis vinifera) that are resistant or tolerant to diseases such as downy mildew (DM). In many crops, the knock-out of Downy Mildew Resistant 6 (DMR6) proved successful in controlling DM-resistance, but the effect of mutations in DMR6 genes is not yet known in grapevine. Today, gene editing serves crop improvement with small and specific mutations while maintaining the genetic background of commercially important clones. Moreover, recent technological advances allowed to produce non-transgenic grapevine clones by regeneration of protoplasts edited with the CRISPR/Cas

SUBMITTER: Giacomelli L 

PROVIDER: S-EPMC10486898 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature