Automated microarray platform for single-cell sorting and collection of lymphocytes following HIV reactivation.

ABSTRACT: A promising strategy to cure HIV-infected individuals is to use latency reversing agents (LRAs) to reactivate latent viruses, followed by host clearance of infected reservoir cells. However, reactivation of latent proviruses within infected cells is heterogeneous and often incomplete. This fact limits strategies to cure HIV which may require complete elimination of viable virus from all cellular reservoirs. For this reason, understanding the mechanism(s) of reactivation of HIV within cellular reservoirs is critical to achieve therapeutic success. Methodologies enabling temporal tracking of single cells as they reactivate followed by sorting and molecular analysis of those cells are urgently needed. To this end, microraft arrays were adapted to image T-lymphocytes expressing mCherry under t

SUBMITTER: Cortes-Llanos B 

PROVIDER: S-EPMC10487311 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature