Dual Modulator of ASIC Channels and GABA_A Receptors from Thyme Alters Fear-Related Hippocampal Activity.

ABSTRACT: Acid-sensing ion channels (ASICs) are proton-gated ion channels that mediate nociception in the peripheral nervous system and contribute to fear and learning in the central nervous system. Sevanol was reported previously as a naturally-occurring ASIC inhibitor from thyme with favorable analgesic and anti-inflammatory activity. Using electrophysiological methods, we found that in the high micromolar range, the compound effectively inhibited homomeric ASIC1a and, in sub- and low-micromolar ranges, positively modulated the currents of α1β2γ2 GABA_A receptors. Next, we tested the compound in anxiety-related behavior models using a targeted delivery into the hippocampus with parallel electroencephalographic measurements. In the open field, 6 µM sevanol reduced both locomotor and θ-rhy

SUBMITTER: Kalinovskii AP 

PROVIDER: S-EPMC10487430 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature