Sequence-based classification scheme for the genus Legionella targeting the mip gene.

ABSTRACT: The identification and speciation of strains of Legionella is often difficult, and even the more successful chromatographic classification techniques have struggled to discriminate newly described species. A sequence-based genotypic classification scheme is reported, targeting approximately 700 nucleotide bases of the mip gene and utilizing gene amplification and direct amplicon sequencing. With the exception of Legionella geestiana, for which an amplicon was not produced, the scheme clearly and unambiguously discriminated among the remaining 39 Legionella species and correctly grouped 26 additional serogroup and reference strains within those species. Additionally, the genotypic classification of approximately 150 wild strains from several continents was consistent with their phenotypic c

SUBMITTER: Ratcliff RM 

PROVIDER: S-EPMC104877 | biostudies-literature | 1998 Jun

REPOSITORIES: biostudies-literature