Proteome-Level Investigation of Vitis amurensis Calli Transformed with a Constitutively Active, Ca²⁺-Independent Form of the Arabidopsis AtCPK1 Gene.

ABSTRACT: Calcium-dependent protein kinases (CDPKs) are one of the main Ca²⁺ decoders in plants. Among them, Arabidopsis thaliana AtCPK1 is one of the most studied CDPK genes as a positive regulator of plant responses to biotic and abiotic stress. The mutated form of AtCPK1, in which the autoinhibitory domain is inactivated (AtCPK1-Ca), provides constitutive kinase activity by mimicking a stress-induced increase in the Ca²⁺ flux. In the present study, we performed a proteomic analysis of Vitis amurensis calli overexpressing the AtCPK1-Ca form using untransformed calli as a control. In our previous studies, we have shown that the overexpression of this mutant form leads to the activation of secondary metabolism in plant cell cultures, including

SUBMITTER: Veremeichik GN 

PROVIDER: S-EPMC10487732 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature