Transcriptome from Paired Samples Improves the Power of Comprehensive COVID-19 Host-Viral Characterization.

ABSTRACT: Previous transcriptome profiling studies showed significantly upregulated genes and altered biological pathways in acute COVID-19. However, changes in the transcriptional signatures during a defined time frame are not yet examined and described. The aims of this study included viral metagenomics and evaluation of the total expression in time-matched and tissue-matched paired COVID-19 samples with the analysis of the host splicing profile to reveal potential therapeutic targets. Prospective analysis of paired nasopharyngeal swabs (NPS) and blood (BL) samples from 18 COVID-19 patients with acute and resolved infection performed using Kallisto, Suppa2, Centrifuge, EdgeR, PantherDB, and L1000CDS2 tools. In NPS, we discovered 6 genes with changed splicing and 40 differentially expressed

SUBMITTER: Milicevic O 

PROVIDER: S-EPMC10487753 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature