A Strainer-Based Platform for the Collection and Immunolabeling of Mouse Intestinal Organoids.

ABSTRACT: Intestinal organoids have emerged as powerful model systems for studying the complex structure and function of the intestine. However, there is a lack of widely applicable methods for the collection, labeling, and imaging of intestinal organoids. In this study, we developed a novel method for loading and labeling intestinal organoids, a method that efficiently collects the organoids and facilitates imaging of their three-dimensional (3D) structure. Based on this strainer platform, mouse intestinal organoids were adequately collected and immobilized, facilitating the immunolabeling workflow to target proteins of the organoids. After evaluation, the strainer size of 40 μm was considered to be more conducive to the collection and labeling of mouse intestinal organoids. More extensive research

SUBMITTER: Tan J 

PROVIDER: S-EPMC10487879 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature