Anionic Phospholipids Stimulate the Proton Pumping Activity of the Plant Plasma Membrane P-Type H⁺-ATPase.

ABSTRACT: The activity of membrane proteins depends strongly on the surrounding lipid environment. Here, we characterize the lipid stimulation of the plant plasma membrane H⁺-ATPase Arabidopsis thaliana H⁺-ATPase isoform 2 (AHA2) upon purification and reconstitution into liposomes of defined lipid compositions. We show that the proton pumping activity of AHA2 is stimulated by anionic phospholipids, especially by phosphatidylserine. This activation was independent of the cytoplasmic C-terminal regulatory domain of the pump. Molecular dynamics simulations revealed several preferential contact sites for anionic phospholipids in the transmembrane domain of AHA2. These contact sites are partially conserved in functionally different P-type ATPases from different organisms, sug

SUBMITTER: Paweletz LC 

PROVIDER: S-EPMC10488199 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature