Interface-Based Design of High-Affinity Affibody Ligands for the Purification of RBD from Spike Proteins.

ABSTRACT: The outbreak of coronavirus disease 2019 (COVID-19) has sparked an urgent demand for advanced diagnosis and vaccination worldwide. The discovery of high-affinity ligands is of great significance for vaccine and diagnostic reagent manufacturing. Targeting the receptor binding domain (RBD) from the spike protein of severe acute respiratory syndrome-coronavirus 2, an interface at the outer surface of helices on the Z domain from protein A was introduced to construct a virtual library for the screening of Z_RBD affibody ligands. Molecular docking was performed using HADDOCK software, and three potential Z_RBD affibodies, Z_RBD-02, Z_RBD-04, and Z_RBD-07, were obtained. Molecular dynamics (MD) simulation verified that the binding of Z_RBD

SUBMITTER: Song S 

PROVIDER: S-EPMC10489752 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature