Examination of ELISA against PCR for assessing treatment efficacy against Cryptosporidium in a clinical trial context.

ABSTRACT:

Background

Cryptosporidium is a gastrointestinal pathogen that presents a serious opportunistic infection in immunocompromised individuals including those living with human immunodeficiency syndrome. The CRYPTOFAZ trial, previously published, was conducted in Malawi to evaluate the efficacy of clofazimine in response to an unmet need for drugs to treat cryptosporidiosis in HIV populations. A combination of rapid diagnostic tests, ELISA, qPCR, and conventional sequencing were employed to detect Cryptosporidium in 586 individuals during pre-screening and monitor oocyst shedding and identify enteric co-pathogens in 22 enrolled/randomized participants during the in-patient period and follow-up visits.

Methodology

Oocyst shedding as measured by qPCR was used to determine primary

SUBMITTER: Nyirenda JT 

PROVIDER: S-EPMC10490871 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature