Exploring the Residue-Level Interactions between the R2ab Protein and Polystyrene Nanoparticles.

ABSTRACT: In biological systems, proteins can bind to nanoparticles to form a "corona" of adsorbed molecules. The nanoparticle corona is of high interest because it impacts the organism's response to the nanomaterial. Understanding the corona requires knowledge of protein structure, orientation, and dynamics at the surface. Ultimately, a residue-level mapping of protein behavior on nanoparticle surfaces is needed, but this mapping is difficult to obtain with traditional approaches. Here, we have investigated the interaction between R2ab and polystyrene nanoparticles (PSNPs) at the level of individual residues. R2ab is a bacterial surface protein from Staphylococcus epidermidis and is known to interact strongly with polystyrene, leading to biofilm formation. We have used mass spectrometry afte

SUBMITTER: Somarathne RP 

PROVIDER: S-EPMC10491123 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature