Shifting Redox Reaction Equilibria on Demand Using an Orthogonal Redox Cofactor.

ABSTRACT: Natural metabolism relies on chemical compartmentalization of two redox cofactors, NAD⁺ and NADP⁺, to orchestrate life-essential redox reaction directions. However, in whole cells the reliance on these canonical cofactors limits flexible control of redox reaction direction as these reactions are permanently tied to catabolism or anabolism. In cell-free systems, NADP⁺ is too expensive in large scale. We have previously reported the use of nicotinamide mononucleotide, (NMN⁺) as a low-cost, noncanonical redox cofactor capable of specific electron delivery to diverse chemistries. Here, we present Nox Ortho, an NMNH-specific water-forming oxidase, that completes the toolkit to modulate NMNH/NMN⁺ ratio. This work uncovers an enzyme design pr

SUBMITTER: Aspacio D 

PROVIDER: S-EPMC10491207 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature