Full-length direct RNA sequencing uncovers stress-granule dependent RNA decay upon cellular stress.

ABSTRACT: Cells react to stress by triggering response pathways, leading to extensive alterations in the transcriptome to restore cellular homeostasis. The role of RNA metabolism in shaping the cellular response to stress is vital, yet the global changes in RNA stability under these conditions remain unclear. In this work, we employ direct RNA sequencing with nanopores, enhanced by 5' end adaptor ligation, to comprehensively interrogate the human transcriptome at single-molecule and nucleotide resolution. By developing a statistical framework to identify robust RNA length variations in nanopore data, we find that cellular stress induces prevalent 5' end RNA decay that is coupled to translation and ribosome occupancy. Unlike typical RNA decay models in normal conditions, we show that stress-induced R

SUBMITTER: Dar SA 

PROVIDER: S-EPMC10491209 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature