Repair of CRISPR-guided RNA breaks enables site-specific RNA editing in human cells.

ABSTRACT: Genome editing with CRISPR RNA-guided endonucleases generates DNA breaks that are resolved by cellular DNA repair machinery. However, analogous methods to manipulate RNA remain unavailable. Here, we show that site-specific RNA breaks generated with RNA-targeting CRISPR complexes are repaired in human cells, and this repair can be used for programmable deletions in human transcripts that restore gene function. Collectively, this work establishes a technology for precise RNA manipulation with potential therapeutic applications.

SUBMITTER: Nemudraia A 

PROVIDER: S-EPMC10491232 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature