A mutant fitness compendium in Bifidobacteria reveals molecular determinants of colonization and host-microbe interactions.

ABSTRACT: Bifidobacteria commonly represent a dominant constituent of human gut microbiomes during infancy, influencing nutrition, immune development, and resistance to infection. Despite interest as a probiotic therapy, predicting the nutritional requirements and health-promoting effects of Bifidobacteria is challenging due to major knowledge gaps. To overcome these deficiencies, we used large-scale genetics to create a compendium of mutant fitness in Bifidobacterium breve (Bb). We generated a high density, randomly barcoded transposon insertion pool in Bb, and used this pool to determine Bb fitness requirements during colonization of germ-free mice and chickens with multiple diets and in response to hundreds of in vitro perturbations. To enable mechanistic invest

SUBMITTER: Shiver AL 

PROVIDER: S-EPMC10491234 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature