Protein Coronas on Functionalized Nanoparticles Enable Quantitative and Precise Large-Scale Deep Plasma Proteomics.

ABSTRACT:

Background

The wide dynamic range of circulating proteins coupled with the diversity of proteoforms present in plasma has historically impeded comprehensive and quantitative characterization of the plasma proteome at scale. Automated nanoparticle (NP) protein corona-based proteomics workflows can efficiently compress the dynamic range of protein abundances into a mass spectrometry (MS)-accessible detection range. This enhances the depth and scalability of quantitative MS-based methods, which can elucidate the molecular mechanisms of biological processes, discover new protein biomarkers, and improve comprehensiveness of MS-based diagnostics.

Methods

Investigating multi-species spike-in experiments and a cohort, we investigated fold-change accuracy, linearity, precision, and s

SUBMITTER: Huang T 

PROVIDER: S-EPMC10491250 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature