Disruption of Synaptic Endoplasmic Reticulum Luminal Protein Containment in <i>Drosophila Atlastin</i> Mutants.

ABSTRACT: The endoplasmic reticulum (ER) extends throughout neurons and regulates many neuronal functions, including neurite outgrowth, neurotransmission, and synaptic plasticity. Mutations in proteins that control ER shape are linked to the neurodegenerative disorder Hereditary Spastic Paraplegia (HSP), yet the ultrastructure and dynamics of neuronal ER remain largely unexplored, especially at presynaptic terminals. Using super-resolution and live imaging in D. melanogaster larval motor neurons, we investigated ER structure at presynaptic terminals of wild-type animals and null mutants of the ER shaping protein and HSP-linked gene, Atlastin. Previous studies using an ER luminal marker reported diffuse localization at Atlastin mutant presynaptic terminals, which was attributed to ER fr

SUBMITTER: Quinones-Frias MC 

PROVIDER: S-EPMC10491308 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature