Melanoma-derived soluble mediators modulate neutrophil biological properties and the release of neutrophil extracellular traps.

ABSTRACT: Polymorphonuclear neutrophils (PMNs) are the main effector cells in the inflammatory response. The significance of PMN infiltration in the tumor microenvironment remains unclear. Metastatic melanoma is the most lethal skin cancer with an increasing incidence over the last few decades. This study aimed to investigate the role of PMNs and their related mediators in human melanoma. Highly purified human PMNs from healthy donors were stimulated in vitro with conditioned media (CM) derived from the melanoma cell lines SKMEL28 and A375 (melanoma CM), and primary melanocytes as controls. PMN biological properties (chemotaxis, survival, activation, cell tracking, morphology and NET release) were evaluated. We found that the A375 cell line produced soluble factors that promoted PMN chemotaxis, surv

SUBMITTER: Modestino L 

PROVIDER: S-EPMC10491523 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature