Structural convergence endows nuclear transport receptor Kap114p with a transcriptional repressor function toward TATA-binding protein.

ABSTRACT: The transcription factor TATA-box binding protein (TBP) modulates gene expression in nuclei. This process requires the involvement of nuclear transport receptors, collectively termed karyopherin-β (Kap-β) in yeast, and various regulatory factors. In previous studies we showed that Kap114p, a Kap-β that mediates nuclear import of yeast TBP (yTBP), modulates yTBP-dependent transcription. However, how Kap114p associates with yTBP to exert its multifaceted functions has remained elusive. Here, we employ single-particle cryo-electron microscopy to determine the structure of Kap114p in complex with the core domain of yTBP (yTBP^C). Remarkably, Kap114p wraps around the yTBP^C N-terminal lobe, revealing a structure resembling transcriptional regulators in complex with TBP, sugg

SUBMITTER: Liao CC 

PROVIDER: S-EPMC10491584 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature